RRM1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI6G8]

CAT#: TA804382AM

RRM1 mouse monoclonal antibody,clone OTI6G8, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RRM1 (RC200726, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RRM1 (1:500). Positive lysates LY422336 (100ug) and LC422336 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from A549 cell line by using anti-RRM1 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804382) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804382) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RRM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804382) (1:500)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI6G8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 541-792 of human RRM1 (NP_001024) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 89.9 kDa
• Gene Name: ribonucleotide reductase catalytic subunit M1
• Database Link:
  NP_001024
  Entrez Gene 20133 MouseEntrez Gene 685579 RatEntrez Gene 6240 Human
  UniProt ID P23921
• Synonyms: R1; RIR1; RR1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways, Purine metabolism, Pyrimidine metabolism