Acid sphingomyelinase (SMPD1) Mouse Monoclonal Antibody [Clone ID: OTI10C5]

CAT#: TA804413S

SMPD1 mouse monoclonal antibody, clone OTI10C5 (formerly 10C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1 (RC219758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMPD1. Positive lysates LY400191 (100ug) and LC400191 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI10C5
• Applications: WB
• Recommended Dilution: WB 1:200
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 62-341 of human SMPD1 (NP_000534) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65 kDa
• Gene Name: sphingomyelin phosphodiesterase 1
• Database Link:
  NP_000534
  Entrez Gene 6609 Human
  UniProt ID P17405
• Background: The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010]
• Synonyms: ASM; ASMASE; NPD

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome, Metabolic pathways, Sphingolipid metabolism