Adiponectin (ADIPOQ) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3G10]

CAT#: TA804768BM

ADIPOQ mouse monoclonal antibody, clone OTI3G10 (formerly 3G10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ADIPOQ (RC229756, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ADIPOQ. Positive lysates LY432756 (100ug) and LC432756 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-ADIPOQ monoclonal antibody at 1:200.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804768)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804768)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804768)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804768)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ADIPOQ mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804768)

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Specifications

Product Data

• Clone Name: OTI3G10
• Applications: IHC, WB
• Recommended Dilution: WB 1:200 - 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-244 of human ADIPOQ (NP_001171271)produced in SF9 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adiponectin, C1Q and collagen domain containing
• Database Link:
  NP_001171271
  Entrez Gene 9370 Human
  UniProt ID Q15848
• Synonyms: ACDC; ACRP30; ADIPQTL1; ADPN; APM-1; APM1; GBP28

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway, Type II diabetes mellitus