NIPP1 (PPP1R8) Mouse Monoclonal Antibody [Clone ID: OTI4E5]

CAT#: TA804812S

PPP1R8 mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R8 (RC210575, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R8. Positive lysates LY408577 (100ug) and LC408577 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPP1R8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804812)

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Specifications

Product Data

• Clone Name: OTI4E5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-225 of human PPP1R8 (NP_612568) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.5 kDa
• Gene Name: protein phosphatase 1 regulatory subunit 8
• Database Link:
  NP_612568
  Entrez Gene 100336 MouseEntrez Gene 313030 RatEntrez Gene 5511 Human
  UniProt ID Q12972
• Background: This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. [provided by RefSeq, Jul
• Synonyms: ARD-1; ARD1; NIPP-1; NIPP1; PRO2047

Reference Data

• Protein Families: Druggable Genome, Transcription Factors