Argininosuccinate Lyase (ASL) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI9G2]

CAT#: TA804902BM

ASL mouse monoclonal antibody,clone OTI9G2, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASL (RC201568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASL (1:2000). Positive lysates LY422562 (100ug) and LC422562 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell line by using anti-CTNND1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and ASL-Knockout Hela cells (KO, Cat# LC810015) were separated by SDS-PAGE and immunoblotted with anti-ASL monoclonal antibody TA804902. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ASL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804902) (1:150) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ASL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804902) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ASL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA804902) (1:500)

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Specifications

Product Data

• Clone Name: OTI9G2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ASL (NP_001020114) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.5 kDa
• Gene Name: argininosuccinate lyase
• Database Link:
  NP_001020114
  Entrez Gene 59085 RatEntrez Gene 109900 MouseEntrez Gene 435 Human
  UniProt ID P04424
• Synonyms: ASAL

Reference Data

• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways