Constitutive androstane receptor (NR1I3) Mouse Monoclonal Antibody [Clone ID: OTI2H10]

CAT#: TA805288

NR1I3 mouse monoclonal antibody, clone OTI2H10 (formerly 2H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3 (RC213905, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NR1I3. Positive lysates LY425868 (100ug) and LC425868 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-NR1I3 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NR1I3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA805288) Dilution: 1:150

Specifications

Product Data

• Clone Name: OTI2H10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-236 of human NR1I3 (NP_001070950) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: nuclear receptor subfamily 1 group I member 3
• Database Link:
  NP_001070950
  Entrez Gene 9970 Human
  UniProt ID Q14994
• Synonyms: CAR; CAR1; MB67

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors