Rb (RB1) Mouse Monoclonal Antibody [Clone ID: OTI11C10]

CAT#: TA805623

RB1 mouse monoclonal antibody,clone OTI11C10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RB1 (RC206933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RB1 mouse monoclonal antibody.1:500`

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-RB1 monoclonal antibody. 1:400

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and RB1-Knockout Hela cells (KO, Cat# LC810199) were separated by SDS-PAGE and immunoblotted with anti-RB1 monoclonal antibody TA805623. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805623) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805623) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805623) (1:500)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805623) (1:500)

• Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-RB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA805623) (1:500)

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Specifications

Product Data

• Clone Name: OTI11C10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 309-590 of human RB1(NP_000312) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RB transcriptional corepressor 1
• Database Link:
  NP_000312
  Entrez Gene 19645 MouseEntrez Gene 24708 RatEntrez Gene 5925 Human
  UniProt ID P06400
• Background: The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
• Synonyms: OSRC; p105-Rb; pp110; PPP1R130; pRb; RB

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Bladder cancer, Cell cycle, Chronic myeloid leukemia, Glioma, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer