SHARP1 (BHLHE41) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5B1]

CAT#: TA806394BM

BHLHE41 mouse monoclonal antibody, clone OTI5B1 (formerly 5B1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHLHE41 (RC206882, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHLHE41. Positive lysates LY410724 (100ug) and LC410724 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BHLHE41 mouse monoclonal antibody. (TA806394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BHLHE41 mouse monoclonal antibody. (TA806394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BHLHE41 mouse monoclonal antibody. (TA806394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BHLHE41 mouse monoclonal antibody. (TA806394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-BHLHE41 mouse monoclonal antibody. (TA806394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-BHLHE41 mouse monoclonal antibody. (TA806394; heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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Specifications

Product Data

• Clone Name: OTI5B1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-297 of human BHLHE41(NP_110389) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.3 kDa
• Gene Name: basic helix-loop-helix family member e41
• Database Link:
  NP_110389
  Entrez Gene 79365 Human
  UniProt ID Q9C0J9
• Background: This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTL's transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. Defects in this gene are associated with the short sleep phenotype. [provided by RefSeq, Feb 2014]
• Synonyms: BHLHB3; DEC2; hDEC2; SHARP1

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal