SMURF 2 (SMURF2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3E4]

CAT#: TA806655BM

SMURF2 mouse monoclonal antibody, clone OTI3E4 (formerly 3E4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMURF2 (RC210866, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMURF2. Positive lysates LY402939 (100ug) and LC402939 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SMURF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806655)

Specifications

Product Data

• Clone Name: OTI3E4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 151-440 of human SMURF2(NP_073576) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86 kDa
• Gene Name: SMAD specific E3 ubiquitin protein ligase 2
• Database Link:
  NP_073576
  Entrez Gene 66313 MouseEntrez Gene 303614 RatEntrez Gene 64750 Human
  UniProt ID Q9HAU4
• Synonyms: DKFZp686F0270; MGC138150

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, TGF-beta signaling pathway, Type I diabetes mellitus, Ubiquitin mediated proteolysis, Viral myocarditis