PD1 (PDCD1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2D10]

CAT#: TA806809AM

PD-1 (PDCD1) mouse monoclonal antibody, clone OTI2D10 (formerly 2D10), Biotinylated

Product Images

Western blot analysis of extracts (35ug) from PBMCs and PHA-stimulated-PBMCs by using anti-PDCD1 monoclonal antibody (1:250).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806809) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA806809) (1:150)

Specifications

Product Data

• Clone Name: OTI2D10
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.2 kDa
• Gene Name: programmed cell death 1
• Database Link:
  NP_005009
  Entrez Gene 5133 Human
  UniProt ID Q15116
• Background: This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
• Synonyms: CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; SLEB2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), T cell receptor signaling pathway