MMP3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2H6]

CAT#: TA806869AM

MMP3 mouse monoclonal antibody, clone OTI2H6 (formerly 2H6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP3 (RC210235, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP3. Positive lysates LY419341 (100ug) and LC419341 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MMP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806869)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MMP3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806869)

Specifications

Product Data

• Clone Name: OTI2H6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 246-477 of human MMP3(NP_002413) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.2 kDa
• Gene Name: matrix metallopeptidase 3
• Database Link:
  NP_002413
  Entrez Gene 4314 Human
  UniProt ID P08254
• Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]
• Synonyms: CHDS6; MMP-3; SL-1; STMY; STMY1; STR1

Reference Data

• Protein Families: Druggable Genome, Protease