c Fos (FOS) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7D6]

CAT#: TA806977BM

FOS mouse monoclonal antibody, clone OTI7D6 (formerly 7D6), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOS (RC202597, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOS (1:4000).

• 

  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-FOS monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806977)

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806977)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806977)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806977)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806977)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FOS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA806977)

Show all 8 images

Specifications

Product Data

• Clone Name: OTI7D6
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FOS (NP_005243) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.5 kDa
• Gene Name: Fos proto-oncogene, AP-1 transcription factor subunit
• Database Link:
  NP_005243
  Entrez Gene 14281 MouseEntrez Gene 314322 RatEntrez Gene 2353 Human
  UniProt ID P01100
• Background: The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
• Synonyms: AP-1; C-FOS; p55

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Colorectal cancer, MAPK signaling pathway, Pathways in cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway