HIPK1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5F1]

CAT#: TA807265BM

HIPK1 mouse monoclonal antibody, clone OTI5F1 (formerly 5F1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HIPK1 (RC206176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HIPK1. Positive lysates LY405750 (100ug) and LC405750 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HIPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807265)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-HIPK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807265)

Specifications

Product Data

• Clone Name: OTI5F1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 439-697 of human HIPK1(NP_852003) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 87.1 kDa
• Gene Name: homeodomain interacting protein kinase 1
• Database Link:
  NP_852003
  Entrez Gene 15257 MouseEntrez Gene 365895 RatEntrez Gene 204851 Human
  UniProt ID Q86Z02
• Background: The protein encoded by this gene belongs to the Ser/Thr family of protein kinases and HIPK subfamily. It phosphorylates homeodomain transcription factors and may also function as a co-repressor for homeodomain transcription factors. Alternative splicing results in four transcript variants encoding four distinct isoforms. [provided by RefSeq, Jul 2008]
• Synonyms: Myak; Nbak2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors