PR3 (PRTN3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI9C2]

CAT#: TA807348AM

PRTN3 mouse monoclonal antibody,clone OTI9C2, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRTN3 (RC220949, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRTN3 (1:500). Positive lysates LY400985 (100ug) and LC400985 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (15ug) from human spleen tissue lysate and mouse brain tissue lysate by using anti-PRTN3 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807348) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807348) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807348) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807348) (1:2000)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PRTN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA807348) (1:2000)

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Specifications

Product Data

• Clone Name: OTI9C2
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 28-248 of human PRTN3(NP_002768) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.6 kDa
• Gene Name: proteinase 3
• Database Link:
  NP_002768
  Entrez Gene 5657 Human
  UniProt ID P24158
• Background: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-. [UniProtKB/Swiss-Prot Function]
• Synonyms: ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP-4; NP4; P29; PR-3; PR3

Reference Data

• Protein Families: Druggable Genome, Protease