SLAMF7 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3B3]

CAT#: TA807414AM

SLAMF7 mouse monoclonal antibody, clone OTI3B3 (formerly 3B3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLAMF7 (RC220985, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLAMF7 (1:2000). Positive lysates LY402852 (100ug) and LC402852 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-SLAMF7 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SLAMF7 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807414) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SLAMF7 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807414) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SLAMF7 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA807414) (1:150)

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  Flow cytometric analysis of living 293T cells transfected with SLAMF7 overexpression plasmid (RC220985), Red)/empty vector (PS100001, Blue) using anti-SLAMF7 antibody (TA807414). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

• Flow cytometric analysis of living OPM2 cells, using anti-SLAMF7 antibody (TA807414, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

• Flow cytometric analysis of living RPMI-8226 cells, using anti-SLAMF7 antibody (TA807414, Red), compared to an isotype control (green), and a PBS control (blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI3B3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLAMF7 (NP_067004) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.2 kDa
• Gene Name: SLAM family member 7
• Database Link:
  NP_067004
  Entrez Gene 57823 Human
  UniProt ID Q9NQ25
• Synonyms: 19A; CD319; CRACC; CS1

Reference Data

• Protein Families: Druggable Genome, Transmembrane