LOXL2 Mouse Monoclonal Antibody [Clone ID: OTI4H3]

CAT#: TA807444S

LOXL2 mouse monoclonal antibody, clone OTI4H3 (formerly 4H3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOXL2 (RC200455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOXL2 (1:2000). Positive lysates LY400844 (100ug) and LC400844 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and LOXL2-Knockout Hela cells (KO, Cat# LC810132) were separated by SDS-PAGE and immunoblotted with anti-LOXL2 monoclonal antibody TA807444. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

Specifications

Product Data

• Clone Name: OTI4H3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 152-450 of human LOXL2(NP_002309) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84 kDa
• Gene Name: lysyl oxidase like 2
• Database Link:
  NP_002309
  Entrez Gene 94352 MouseEntrez Gene 290350 RatEntrez Gene 4017 Human
  UniProt ID Q9Y4K0
• Background: This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]
• Synonyms: LOR2; WS9-14

Reference Data

• Protein Families: Druggable Genome, Secreted Protein