TRIB3 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3C6]

CAT#: TA807483AM

TRIB3 mouse monoclonal antibody, clone OTI3C6 (formerly 3C6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIB3 (RC206687, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIB3. Positive lysates LY402848 (100ug) and LC402848 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807483)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807483)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807483)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-TRIB3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807483)

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Specifications

Product Data

• Clone Name: OTI3C6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-200 of human TRIB3(NP_066981) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.4 kDa
• Gene Name: tribbles pseudokinase 3
• Database Link:
  NP_066981
  Entrez Gene 57761 Human
  UniProt ID Q96RU7
• Background: The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. [provided by RefSeq, Jul 2008]
• Synonyms: C20orf97; NIPK; SINK; SKIP3; TRB3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors