WDR61 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4C4]

CAT#: TA807587BM

WDR61 mouse monoclonal antibody, clone OTI4C4 (formerly 4C4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR61 (RC203963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR61. Positive lysates LY410745 (100ug) and LC410745 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-WDR61 monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)at 1:200 dilution.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807587)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807587)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807587)

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Specifications

Product Data

• Clone Name: OTI4C4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 92-305 of human WDR61(NP_079510) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.4 kDa
• Gene Name: WD repeat domain 61
• Database Link:
  NP_079510
  Entrez Gene 66317 MouseEntrez Gene 363064 RatEntrez Gene 80349 Human
  UniProt ID Q9GZS3
• Background: WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]). [supplied by OMIM, Mar 2008]
• Synonyms: REC14; SKI8

Reference Data

• Protein Pathways: RNA degradation