MED15 Mouse Monoclonal Antibody [Clone ID: OTI3H10]

CAT#: TA807884S

MED15 mouse monoclonal antibody,clone OTI3H10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MED15 (RC204224, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MED15 (1:2000). Positive lysates LY402473 (100ug) and LC402473 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-MED15 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI3H10
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 393-679 of human MED15(NP_056973) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 82.4 kDa
• Gene Name: mediator complex subunit 15
• Database Link:
  NP_056973
  Entrez Gene 51586 Human
  UniProt ID Q96RN5
• Background: The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
• Synonyms: ARC105; CAG7A; CTG7A; PCQAP; TIG-1; TIG1; TNRC7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors