NOTCH1 Mouse Monoclonal Antibody [Clone ID: OTI2E7]

CAT#: TA808308S

NOTCH1 mouse monoclonal antibody, clone OTI2E7 (formerly 2E7)

Product Images

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NOTCH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA808308) (1:150)

Specifications

Product Data

• Clone Name: OTI2E7
• Applications: IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2280-2556 of human NOTCH1(NP_060087) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 270.6 kDa
• Gene Name: notch 1
• Database Link:
  NP_060087
  Entrez Gene 18128 MouseEntrez Gene 25496 RatEntrez Gene 4851 Human
  UniProt ID P46531
• Background: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. [provided by RefSeq, Jul 2008]
• Synonyms: AOS5; AOVD1; hN1; TAN1

Reference Data

• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway
• Protein Pathways: Dorso-ventral axis formation, Notch signaling pathway, Prion diseases