SPATA22 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1E2]

CAT#: TA808425AM

SPATA22 mouse monoclonal antibody, clone OTI1E2 (formerly 1E2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPATA22 (RC205958, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPATA22 (1:2000). Positive lysates LY410013 (100ug) and LC410013 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808425) (1:150).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808425) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808425) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808425) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SPATA22 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808425) (1:150)

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Specifications

Product Data

• Clone Name: OTI1E2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SPATA22(NP_115987) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.1 kDa
• Gene Name: spermatogenesis associated 22
• Database Link:
  NP_115987
  Entrez Gene 84690 Human
  UniProt ID Q8NHS9
• Background: Meiosis-specific protein required for homologous recombination in meiosis I. [UniProtKB/Swiss-Prot Function]
• Synonyms: NYD-SP20; NYDSP20