SCFD1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI10D10]

CAT#: TA808451AM

SCFD1 mouse monoclonal antibody,clone OTI10D10, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCFD1 (RC217990, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SCFD1 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SCFD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808451) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SCFD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808451) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SCFD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808451) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SCFD1 mouse monoclonal antibody.This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808451) (1:2000)

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Specifications

Product Data

• Clone Name: OTI10D10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 66-190 of human SCFD1(NP_878255) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sec1 family domain containing 1
• Database Link:
  NP_878255
  Entrez Gene 54350 RatEntrez Gene 76983 MouseEntrez Gene 23256 Human
  UniProt ID Q8WVM8
• Background: Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi. [UniProtKB/Swiss-Prot Function]
• Synonyms: C14orf163; RA410; SLY1; SLY1P; STXBP1L2