TFIIB (GTF2B) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2F5]

CAT#: TA808573BM

GTF2B mouse monoclonal antibody, clone OTI2F5 (formerly 2F5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B (1:2000). Positive lysates LY400582 (100ug) and LC400582 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 10 different cell lines by using anti-GTF2B monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI2F5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 43-316 of human GTF2B(NP_001505) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.7 kDa
• Gene Name: general transcription factor IIB
• Database Link:
  NP_001505
  Entrez Gene 81673 RatEntrez Gene 229906 MouseEntrez Gene 2959 Human
  UniProt ID Q00403
• Background: This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
• Synonyms: TF2B; TFIIB

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Basal transcription factors