Cyclophilin F (PPIF) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C4]

CAT#: TA809029AM

PPIF mouse monoclonal antibody,clone OTI1C4, Biotinylated

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPIF-Knockout 293T cells (KO, Cat# LC811541) were separated by SDS-PAGE and immunoblotted with anti-PPIF monoclonal antibody TA809029, (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809029) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809029) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809029) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809029) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PPIF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809029) (1:150)

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Specifications

Product Data

• Clone Name: OTI1C4
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPIF(NP_005720) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.8 kDa
• Gene Name: peptidylprolyl isomerase F
• Database Link:
  NP_005720
  Entrez Gene 105675 MouseEntrez Gene 282819 RatEntrez Gene 10105 Human
  UniProt ID P30405
• Background: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. [provided by RefSeq, Jul 2008]
• Synonyms: Cyp-D; CyP-M; CYP3; CypD