S100A5 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4C9]

CAT#: TA809056AM

S100A5 mouse monoclonal antibody, clone OTI4C9 (formerly 4C9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY S100A5 (RC210891, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-S100A5 (1:2000). Positive lysates LY418992 (100ug) and LC418992 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4C9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human S100A5 (NP_002953) produced in 293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.6 kDa
• Gene Name: S100 calcium binding protein A5
• Database Link:
  NP_002953
  Entrez Gene 20199 MouseEntrez Gene 295211 RatEntrez Gene 6276 Human
  UniProt ID P33763
• Background: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. [provided by RefSeq, Jul 2008]
• Synonyms: S100D