NCF4 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3E8]

CAT#: TA809222BM

NCF4 mouse monoclonal antibody,clone OTI3E8, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NCF4 (RC216094, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NCF4 (1:500). Positive lysates LY424600 (100ug) and LC424600 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3E8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NCF4 (NP_000622) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.9 kDa
• Gene Name: neutrophil cytosolic factor 4
• Database Link:
  NP_000622
  Entrez Gene 17972 MouseEntrez Gene 500904 RatEntrez Gene 4689 Human
  UniProt ID Q15080
• Background: The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
• Synonyms: NCF; P40PHOX; SH3PXD4

Reference Data

• Protein Pathways: Leukocyte transendothelial migration