IRF3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8G9]

CAT#: TA809316BM

IRF3 mouse monoclonal antibody,clone OTI8G9, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF3 (RC209951, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF3 (1:500).

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  Western blot analysis of extracts (35ug) from 5 different cell lines and human plasma by using anti-IRF3 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and IRF3-Knockout HeLa cells (KO, Cat# LC833375 ) were separated by SDS-PAGE and immunoblotted with anti-IRF3 monoclonal antibody TA809316 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY IRF3 (RC209951) using anti-IRF3 antibody (TA809316/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

Specifications

Product Data

• Clone Name: OTI8G9
• Applications: IF, WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 27-169 of human IRF3 (NP_001184053) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interferon regulatory factor 3
• Database Link:
  NP_001184053
  Entrez Gene 3661 Human
  UniProt ID Q14653
• Background: This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]
• Synonyms: IIAE7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway