n-Myc (MYCN) Mouse Monoclonal Antibody [Clone ID: OTI2E5]

CAT#: TA809374

MYCN mouse monoclonal antibody,clone OTI2E5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYCN (RC201241, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYCN (1:500). Positive lysates LY417334 (100ug) and LC417334 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-MYCN monoclonal antibody (HepG2: human; HeLa: human; RAMOS: human) (1:500).

Specifications

Product Data

• Clone Name: OTI2E5
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 229-464 of human MYCN(NP_005369) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.4 kDa
• Gene Name: v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog
• Database Link:
  NP_005369
  Entrez Gene 4613 Human
  UniProt ID P04198
• Background: This gene is a member of the MYC family and encodes a protein with a basic helix-loop-helix (bHLH) domain. This protein is located in the nucleus and must dimerize with another bHLH protein in order to bind DNA. Amplification of this gene is associated with a variety of tumors, most notably neuroblastomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
• Synonyms: bHLHe37; MODED; N-myc; NMYC; ODED

Reference Data

• Protein Families: Druggable Genome, Transcription Factors