AGR2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5D8]

CAT#: TA809755AM

AGR2 mouse monoclonal antibody,clone OTI5D8, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGR2 (RC202023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGR2 (1:2000). Positive lysates LY416668 (100ug) and LC416668 (20ug) can be purchased separately from OriGene.

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  HEK293T cells were transfected with the pCMV6-ENTRY AGR2 (RC202023, Left lane) or pCMV6-ENTRY AGR3 (RC208897, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGR2 (1:2000).

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  Western blot analysis of extracts (35ug) from 3 different cell lines by using anti-AGR2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AGR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809755) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-AGR2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809755) (1:2000)

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Specifications

Product Data

• Clone Name: OTI5D8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-175 of human AGR2(NP_006399) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.8 kDa
• Gene Name: anterior gradient 2, protein disulphide isomerase family member
• Database Link:
  NP_006399
  Entrez Gene 23795 MouseEntrez Gene 298961 RatEntrez Gene 10551 Human
  UniProt ID O95994
• Synonyms: AG2; GOB-4; HAG-2; HEL-S-116; PDIA17; XAG-2

Reference Data

• Protein Families: Secreted Protein