P Glycoprotein (ABCB1) Mouse Monoclonal Antibody [Clone ID: OTI9C10]

CAT#: TA809814S

ABCB1 mouse monoclonal antibody,clone OTI9C10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCB1 (RC216080, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABCB1 (1:2000).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809814) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ABCB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809814) (1:2000)

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  Flow cytometric Analysis of permeabilized Hek293T cells, using anti-ABCB1 antibody (TA809814), (Red), compared to negative control (PBS), (Blue). (1:100).

Specifications

Product Data

• Clone Name: OTI9C10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 995-1280 of human ABCB1 (NP_000918) produced in SF9 cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATP binding cassette subfamily B member 1
• Database Link:
  NP_000918
  Entrez Gene 170913 RatEntrez Gene 5243 Human
  UniProt ID P08183
• Background: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]
• Synonyms: ABC20; CD243; CLCS; GP170; MDR1; P-GP; PGY1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: ABC transporters