SIRT1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1H1]

CAT#: TA809831AM

SIRT1 mouse monoclonal antibody,clone OTI1H1, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT1 (RC218134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT1 (1:1000).

• 

  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-SIRT1 monoclonal antibody (1:500).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and SIRT1-Knockout Hela cells (KO, Cat# LC810336) were separated by SDS-PAGE and immunoblotted with anti-SIRT1 monoclonal antibody TA809831. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

Specifications

Product Data

• Clone Name: OTI1H1
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 552-676 of human SIRT1 (NP_036370) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.5 kDa
• Gene Name: sirtuin 1
• Database Link:
  NP_036370
  Entrez Gene 93759 MouseEntrez Gene 309757 RatEntrez Gene 23411 Human
  UniProt ID Q96EB6
• Synonyms: SIR2; SIR2alpha; SIR2L1

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors