CBR1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4B4]

CAT#: TA809840BM

CBR1 mouse monoclonal antibody,clone OTI4B4, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CBR1 (RC204950, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CBR1 (1:2000). Positive lysates LY400669 (100ug) and LC400669 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different tissue lysates by using anti-CBR1 monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-CBR1 monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type A549 cells and CBR1-Knockout A549 cells (KO, Cat# LC800036) were separated by SDS-PAGE and immunoblotted with anti-CBR1 monoclonal antibody TA809840 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CBR1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809840) (1:2000)

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Specifications

Product Data

• Clone Name: OTI4B4
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CBR1 (NP_001748) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.2 kDa
• Gene Name: carbonyl reductase 1
• Database Link:
  NP_001748
  Entrez Gene 12408 MouseEntrez Gene 29224 RatEntrez Gene 873 Human
  UniProt ID P16152
• Background: The protein encoded by this gene belongs to the short-chain dehydrogenases/reductases (SDR) family, which function as NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds, such as quinones, prostaglandins, and various xenobiotics. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2013]
• Synonyms: CBR; hCBR1; SDR21C1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways