Semaphorin 4D (SEMA4D) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI8E6]

CAT#: TA809845AM

SEMA4D mouse monoclonal antibody,clone OTI8E6, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEMA4D (RC208521, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEMA4D (1:2000). Positive lysates LY416686 (100ug) and LC416686 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SEMA4D mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA809845) (1:500)

Specifications

Product Data

• Clone Name: OTI8E6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SEMA4D (NP_006369) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96 kDa
• Gene Name: semaphorin 4D
• Database Link:
  NP_006369
  Entrez Gene 10507 Human
  UniProt ID Q92854
• Synonyms: C9orf164; CD100; coll-4; M-sema-G; SEMAJ

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance