PSMD9 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5H2]

CAT#: TA810188BM

PSMD9 mouse monoclonal antibody,clone OTI5H2, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD9 (RC200903, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD9 (1:2000). Positive lysates LY419093 (100ug) and LC419093 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 5 different cell lines by using anti-PSMD9 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI5H2
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PSMD9 (NP_002804) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.5 kDa
• Gene Name: proteasome 26S subunit, non-ATPase 9
• Database Link:
  NP_002804
  Entrez Gene 161475 RatEntrez Gene 5715 Human
  UniProt ID O00233
• Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012]
• Synonyms: p27; Rpn4