SHARP2 (BHLHE40) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI10H1]

CAT#: TA810228AM

BHLHE40 mouse monoclonal antibody,clone OTI10H1, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BHLHE40 (RC210294, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BHLHE40 (1:2000). Positive lysates LY418509 (100ug) and LC418509 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BHLHE40 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810228) (1:150)

Specifications

Product Data

• Clone Name: OTI10H1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-313 of human BHLHE40 (NP_003661) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45.3 kDa
• Gene Name: basic helix-loop-helix family member e40
• Database Link:
  NP_003661
  Entrez Gene 20893 MouseEntrez Gene 79431 RatEntrez Gene 8553 Human
  UniProt ID O14503
• Synonyms: BHLHB2; DEC1; HLHB2; SHARP-2; STRA13; Stra14

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Circadian rhythm - mammal