AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI2A3]

CAT#: TA810438S

AFAP1 mouse monoclonal antibody,clone OTI2A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC207421, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1 (1:2000). Positive lysates LY402869 (100ug) and LC402869 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AFAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810438) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-AFAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810438) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-AFAP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810438) (1:2000)

Specifications

Product Data

• Clone Name: OTI2A3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AFAP1 (NP_067651) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.5 kDa
• Gene Name: actin filament associated protein 1
• Database Link:
  NP_067651
  Entrez Gene 70292 MouseEntrez Gene 140935 RatEntrez Gene 60312 Human
  UniProt ID Q8N556
• Synonyms: 110 kDa; actin filament-associated protein; actin filament associated protein 1; AFAP; AFAP-110; FLJ56849; OTTHUMP00000155170