Calcineurin A (PPP3CA) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5C6]

CAT#: TA810584BM

PPP3CA mouse monoclonal antibody,clone OTI5C6, HRP conjugated

Product Images

Western blot analysis of extracts (35ug) from cell lines by using anti-PPP3CA monoclonal antibody (1:500).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PPP3CA-Knockout HeLa cells (KO, Cat# LC831038) were separated by SDS-PAGE and immunoblotted with anti-PPP3CA monoclonal antibody TA810584 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

• 

  Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-PPP3CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810584) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-PPP3CA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810584) (1:2000)

Specifications

Product Data

• Clone Name: OTI5C6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-105 of human PPP3CA (NP_000935) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein phosphatase 3 catalytic subunit alpha
• Database Link:
  NP_000935
  Entrez Gene 19055 MouseEntrez Gene 24674 RatEntrez Gene 5530 Human
  UniProt ID Q08209
• Synonyms: ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; IECEE; IECEE1; PPP2B

Reference Data

• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway