FOXO1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2B5]

CAT#: TA810652AM

FOXO1 mouse monoclonal antibody,clone OTI2B5, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXO1 (RC200477, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXO1 (1:2000). Positive lysates LY419586 (100ug) and LC419586 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FOXO1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810652) (1:150)

Specifications

Product Data

• Clone Name: OTI2B5
• Applications: WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 356-655 of human FOXO1 (NP_002006) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 69.5 kDa
• Gene Name: forkhead box O1
• Database Link:
  NP_002006
  Entrez Gene 56458 MouseEntrez Gene 84482 RatEntrez Gene 2308 Human
  UniProt ID Q12778
• Synonyms: FKH1; FKHR; FOXO1A

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Insulin signaling pathway, Pathways in cancer, Prostate cancer