PD L1 (CD274) Mouse Monoclonal Antibody [Clone ID: OTI9E10]

CAT#: TA810744

CD274 mouse monoclonal antibody,clone OTI9E10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (RC213071, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500). Positive lysates LY415473 (100ug) and LC415473 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cancer cell lines by using anti-CD274 monoclonal antibody (1:500).

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  Living HEK293T cells transfected with either RC213071 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD274 antibody (TA810744), and then analyzed by flow cytometry (1:100).

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  Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody (TA810744), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100).

Specifications

Product Data

• Clone Name: OTI9E10
• Applications: FC, WB
• Recommended Dilution: FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-290 of human CD274 (NP_054862) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.28 kDa
• Gene Name: CD274 molecule
• Database Link:
  NP_054862
  Entrez Gene 60533 MouseEntrez Gene 29126 Human
  UniProt ID Q9NZQ7
• Synonyms: B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)