ELL Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3A8]

CAT#: TA810824BM

ELL mouse monoclonal antibody,clone OTI3A8, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELL (RC208258, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELL (1:2000). Positive lysates LY416583 (100ug) and LC416583 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines and mouse testis tissue lysate by using anti-ELL monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ELL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810824) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ELL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810824) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-ELL mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810824) (1:150)

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Specifications

Product Data

• Clone Name: OTI3A8
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 302-621 of human ELL (NP_006523) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.1 kDa
• Gene Name: elongation factor for RNA polymerase II
• Database Link:
  NP_006523
  Entrez Gene 13716 MouseEntrez Gene 8178 Human
  UniProt ID P55199
• Synonyms: C19orf17; ELL1; MEN; PPP1R68

Reference Data

• Protein Families: Transcription Factors