ASH2L Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2H3]

CAT#: TA810988BM

ASH2L mouse monoclonal antibody,clone OTI2H3, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASH2L (RC215552, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASH2L (1:2000). Positive lysates LY401483 (100ug) and LC401483 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 cell lines lysates by using anti-ASH2L monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ASH2L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810988) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ASH2L mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA810988) (1:2000)

Specifications

Product Data

• Clone Name: OTI2H3
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 347-628 of human ASH2L (NP_004665) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.5 kDa
• Gene Name: ASH2 like histone lysine methyltransferase complex subunit
• Database Link:
  NP_004665
  Entrez Gene 23808 MouseEntrez Gene 290829 RatEntrez Gene 9070 Human
  UniProt ID Q9UBL3
• Synonyms: ASH2; ASH2L1; ASH2L2; Bre2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors