TIMM23 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2F5]

CAT#: TA811032AM

TIMM23 mouse monoclonal antibody,clone OTI2F5, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIMM23 (RC209369, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIMM23 (1:2000). Positive lysates LY401917 (100ug) and LC401917 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-TIMM23 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TIMM23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811032) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TIMM23 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811032) (1:2000)

• 

  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-TIMM23 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:2000)

Show all 5 images

Specifications

Product Data

• Clone Name: OTI2F5
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human TIMM23 (NP_006318) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.8 kDa
• Gene Name: translocase of inner mitochondrial membrane 23
• Database Link:
  NP_006318
  Entrez Gene 100287932 Human
  UniProt ID O14925
• Synonyms: TIM23