Septin 3 (SEPT3) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3H6]

CAT#: TA811196AM

SEPT3 mouse monoclonal antibody,clone OTI3H6, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT3 (RC215771, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT3 (1:2000). Positive lysates LY403438 (100ug) and LC403438 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 293T cell line and mouse brain tissue lysate by using anti-SEPT3 monoclonal antibody (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SEPT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811196) (1:500)

• 

  Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-SEPT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)

Specifications

Product Data

• Clone Name: OTI3H6
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombiant protein of human SEPT3 (NP_663786) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.5 kDa
• Gene Name: septin 3
• Database Link:
  NP_663786
  Entrez Gene 24050 MouseEntrez Gene 55964 Human
  UniProt ID Q9UH03
• Synonyms: bK250D10.3; SEP3