YBX2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3C12]

CAT#: TA811247BM

YBX2 mouse monoclonal antibody,clone OTI3C12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY YBX2 (RC208392, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-YBX2 (1:2000). Positive lysates LY414272 (100ug) and LC414272 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from mouse testis tissue lysate by using anti-YBX2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-YBX2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811247) (1:500)

Specifications

Product Data

• Clone Name: OTI3C12
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human YBX2 (NP_057066) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Y-box binding protein 2
• Database Link:
  NP_057066
  Entrez Gene 53422 MouseEntrez Gene 303250 RatEntrez Gene 51087 Human
  UniProt ID Q9Y2T7
• Background: This gene encodes a nucleic acid binding protein which is highly expressed in germ cells. The encoded protein binds to a Y-box element in the promoters of certain genes but also binds to mRNA transcribed from these genes. Pseudogenes for this gene are located on chromosome 10 and 15. [provided by RefSeq, Feb 2012]
• Synonyms: CONTRIN; CSDA3; DBPC; MSY2