CAS9 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C8]

CAT#: TA811327AM

CAS9 mouse monoclonal antibody,clone OTI1C8, Biotinylated

Product Images

Immunoprecipitation (IP) of Cas9 and Cas9-nickase by using mouse monoclonal anti-CAS9 antibodies TA811194 and TA811327. Mouse IgG control serves as the negative control. 293T cells were transfected with flag-tagged Cas9 overexpression plasmid, pCas-Guide (G) and pCas-Guide-nickase (N). Overexpression cell lysates were first precleared with ProteinG beads then incubated with beads crosslinked with anti-CAS9 antibody for overnight. The beads were then rinced with buffer and went through Western Blot analysis using anti-flag antibody (TA180144) (1:30).

Specifications

Product Data

• Clone Name: OTI1C8
• Applications: IP
• Recommended Dilution: IP 1:30
• Reactivities: Streptococcus Pyogenes
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-1166 of human CAS9 produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.