FHIT Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1D7]

CAT#: TA811440AM

FHIT mouse monoclonal antibody,clone OTI1D7, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHIT (RC207120, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHIT (1:2000). Positive lysates LY419588 (100ug) and LC419588 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FHIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811440) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FHIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811440) (1:250)

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  Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-FHIT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811440) (1:250)

Specifications

Product Data

• Clone Name: OTI1D7
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:250
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FHIT (NP_002003) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 16.7 kDa
• Gene Name: fragile histidine triad
• Database Link:
  NP_002003
  Entrez Gene 14198 MouseEntrez Gene 60398 RatEntrez Gene 2272 Human
  UniProt ID P49789
• Synonyms: AP3Aase; FRA3B

Reference Data

• Protein Pathways: Non-small cell lung cancer, Purine metabolism, Small cell lung cancer