PPP2R1B Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8C5]

CAT#: TA811459BM

PPP2R1B mouse monoclonal antibody,clone OTI8C5, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP2R1B (RC230265, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP2R1B (1:2000). Positive lysates LY433265 (100ug) and LC433265 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 different cell lines by using anti-PPP2R1B monoclonal antibody (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPP2R1B-Knockout 293T cells (KO, Cat# LC811012) were separated by SDS-PAGE and immunoblotted with anti-PPP2R1B monoclonal antibody TA811459, (1:500). Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control.

Specifications

Product Data

• Clone Name: OTI8C5
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 19-601 of human PPP2R1B (NP_859050) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: protein phosphatase 2 scaffold subunit Abeta
• Database Link:
  NP_001171033
  Entrez Gene 73699 MouseEntrez Gene 315648 RatEntrez Gene 5519 Human
  UniProt ID P30154
• Synonyms: PP2A-Abeta; PR65B

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway