KLC2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI10H10]

CAT#: TA811478AM

KLC2 mouse monoclonal antibody,clone OTI10H10, Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KLC2 (RC214206, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KLC2 (1:2000). Positive lysates LY402950 (100ug) and LC402950 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from HELA cell line by using anti-KLC2 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-KLC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811478) (1:2000)

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  Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-KLC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA811478) (1:2000)

Specifications

Product Data

• Clone Name: OTI10H10
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 335-622 of human KLC2 (NP_073733) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: kinesin light chain 2
• Database Link:
  NP_073733
  Entrez Gene 64837 Human
  UniProt ID Q9H0B6
• Background: Kinesin is a molecular motor that generates ATP-dependent movement of vesicles and organelles along microtubules. Kinesin consists of 2 light chains, such as KLC2, and 2 heavy chains (see KIF5B; MIM 602809) in a 1:1 stoichiometric ratio (Rahman et al., 1998 [PubMed 9624122]). [supplied by OMIM, Mar 2008]
• Synonyms: SPOAN

Reference Data

• Protein Families: Druggable Genome