WIPI1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI10F4]

CAT#: TA811509BM

WIPI1 mouse monoclonal antibody,clone OTI10F4, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WIPI1 (RC208094, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WIPI1 (1:2000). Positive lysates LY402635 (100ug) and LC402635 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-WIPI1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI10F4
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 195-446 of human WIPI1 (NP_060453) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.5 kDa
• Gene Name: WD repeat domain, phosphoinositide interacting 1
• Database Link:
  NP_060453
  Entrez Gene 52639 MouseEntrez Gene 303630 RatEntrez Gene 55062 Human
  UniProt ID Q5MNZ9
• Background: This gene encodes a WD40 repeat protein. Members of the WD40 repeat family are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
• Synonyms: ATG18; ATG18A; WIPI49