REV1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1E12]

CAT#: TA811575BM

REV1 mouse monoclonal antibody,clone OTI1E12, HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY REV1 (RC221742, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-REV1 (1:2000). Positive lysates LY402538 (100ug) and LC402538 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-REV1 monoclonal antibody (1:500).

Specifications

Product Data

• Clone Name: OTI1E12
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1079-1251 of human REV1 (NP_057400) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: REV1, DNA directed polymerase
• Database Link:
  NP_057400
  Entrez Gene 316344 RatEntrez Gene 51455 Human
  UniProt ID Q9UBZ9
• Background: This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. [provided by RefSeq, Mar 2016]
• Synonyms: AIBP80; REV1L

Reference Data

• Protein Families: Druggable Genome